Movie 20

Cdc42_FIL:WT sprout
Simulation movie showing a 1:1 Cdc42_FIL:WT sprout in which the green and red cells respectively depict Cdc42_FIL and WT cells. The green Cdc42_FIL mutant cells extend less filopodia and are less competent to occupy the tip position than the red WT cells. The Cdc42_FIL and WT cells segregate vertically, with the Cdc42_FIL and WT cells respectively at the top and bottom of the vessel sprout, and this in a persistent way (compare with Cdc42_POL:WT sprout in Movie 22). They additionally segregate horizontally, with the Cdc42_FIL cells at the rear of the sprout, albeit in a rather transient way (compare with Cdc42_POL:WT sprout in Movie 22). The green layer underneath the vessel shows the VEGF gradient, which linearly increases towards the tip.