Simulation movie showing a 1:1 Cdc42FIL:WT sprout in which the green and red cells respectively depict Cdc42FIL and WT cells. The green Cdc42FIL mutant cells extend less filopodia and are less competent to occupy the tip position than the red WT cells. The Cdc42FIL and WT cells segregate vertically, with the Cdc42FIL and WT cells respectively at the top and bottom of the vessel sprout, and this in a persistent way (compare with Cdc42POL:WT sprout in Movie 22). They additionally segregate horizontally, with the Cdc42FIL cells at the rear of the sprout, albeit in a rather transient way (compare with Cdc42POL:WT sprout in Movie 22). The green layer underneath the vessel shows the VEGF gradient, which linearly increases towards the tip.