Movie 21

Vertical segregation of Cdc42KO-EC in vivo
Endothelial cells lacking Cdc42 preferentially occupy the upper side (facing the vitreous) of growing blood vessels and are underrepresented on the bottom side of vessels (facing the astrocytes). 3-D animation of a XYZ Confocal stack, showing a partially recombined vein in a P7 Cdc42^iΔEC retina. Cdc42KO cells are indicated by YFP expression (green), ECs are visualized by CD31 staining (red).