Movie 22

Cdc42_POL:WT sprout
Simulation movie showing a 1:1 Cdc42_POL:WT sprout in which the green and red cells respectively depict Cdc42_POL and WT cells. The green Cdc42_POL cells are less polarized and are less competent to occupy the tip position than the red WT cells. The Cdc42_POL and WT cells segregate vertically, with the Cdc42_POL and WT cells respectively at the top and bottom of the vessel sprout, albeit only transiently (compare with Cdc42_FIL:WT sprout in Movie 21). In contrast, the horizontal segregation of the Cdc42_POL and WT cells, with the Cdc42_POL cells at the rear of the sprout, occurs in a more persistent way (compare with Cdc42_FIL:WT sprout in Movie 21). The green layer underneath the vessel shows the VEGF gradient, which linearly increases towards the tip.