Vkg-GFP signal in a cultured pupal wing disc. The movie shows the eversion of the disc, retraction of the peripodial membrane and elongation of the wing primordium. See that Vkg-GFP is present in the wing pouch early (green arrowhead) but not after eversion of the disc (white arrowhead). In this and subsequent movies, numbers indicate hours in the movie, which, however, do not exactly correspond to developmental hours in the pupa. All the movies start approximately at 3h APF.