Movie 3

In kinase-dead Pak1 expressing aggregates cells overcome contact inhibition of lamellipodia. A time lapse recording of a KD-Pak1 expressing aggregate (green, mb-GFP) is shown side by side with an untreated aggregate (red, mb-RFP) on a FN coated dish. The KDPak1 expressing cells show an increased number of large protrusions with an increased survival time. Untreated cells in the aggregate are more densely packed and have only small short lived protrusions.

PDGF-A suppresses contact inhibition during directional collective cell migration

Martina Nagel, and Rudolf Winklbauer

Development 2018. 145:None-None; doi: 10.1242/dev.162651