Movie 1

Gpc4 is required for efficient endoderm C&E during early segmentation. Time-lapse experiments were performed on Tg(sox17:EGFP) control or gpc4 mutant embryos from 1-6s, using an epifluorescence microscope (DMI 6000, Leica) with a 5x/NA 0.15 objective. Images were acquired at 5-min intervals and movie plays at 5 frames/sec.