Movie 3

Rac1 activity in migrating endodermal cells in control and gpc4 mutant embryos.
Representative time-lapse movies of anterior endoderm in control (left panel) or gpc4 mutant (right panel) host embryo transplanted with sox32, PDB-GFP-expressing and rhodamine-labeled control (left panel) or gpc4 mutant (right panel) cells. Images were acquired at 10-sec intervals using a Ziess LSM700 confocal microscope with a LD C-Apo 40×/NA 1.1 water objective and movie plays at 5 frames/sec.