Movie 4

C&E of gpc4-deficient donor cells in wild-type host embryo.
Representative time-lapse movie of anterior endoderm of a Tg(sox17:EGFP) embryo transplanted with sox32-expressing, rhodamine-labeled gpc4-deficient cells (magenta), from 2-5s. Images were captured at 5-min intervals using an epifluorescence microscope (DMI 6000, Leica) with a 5x/NA 0.15 objective. The movie plays at 5 frames/sec.