Movie 1

3-Dimensional view of the pseudo-temporal trajectory analysis of the fetal kidney single cell expression data. Single cell RNASeq data from 5 human fetal kidney datasets (87, 105, 110, 115, and 132 days old) were used in this analysis. Each developmental trajectory is color-coded and each dot represents a cell. Cells with > 1000 genes /cell and < 25% mitochondrial read content were used this cell-ordering analysis. 3 independent developmental trajectories were identified: the stroma, the collecting duct and the nephron lineage. The proximity between the stromal trajectory and the nephron progenitor trajectory reflects a significant degree of gene expression overlap. Similarly, the proximity between the collecting duct and the distal/connecting tubules trajectory reflects the expected proximity in gene expression, as well as function between these two closely located segments, despite originating from distinct renal compartments, the UB and the nephron progenitors respectively.