Cortical confocal spinning disk imaging of PIP2 monitored by mNeonGreen::PHPLC1δ1 (green) and of F-actin monitored by Lifeact::mKate-2 (red) upon pfn-1(RNAi) with slower frame rate than Movie 9. Note the slight back and forth movement with occasional jumps, in contrast to the directed movement of PIP2 cortical structures and F-actin. Scale bar: 5 μm.