To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 20 Middle plane confocal spinning disk imaging of a perm-1(RNAi) embryo, expressing mCherry::PHPLC1δ1 and GFP::PAR-2 upon Ionomycin/Ca2+ and Latrunculin D treatment. Note embryo movement caused by flow of the buffer after Latrunculin A addition. PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos Melina J. Scholze, Kévin S. Barbieux, Alessandro De Simone, Mathilde Boumasmoud, Camille C. N. Süess, Ruijia Wang, and Pierre Gönczy Development 2018. 145:None-None; doi: 10.1242/dev.164988 Download Original Video