Movie 16

Timelapse of ptch2^tc294z mutant donor cells in a ptch2^tc294z mutant host. At the start of the movie, donor cells (magenta) are found within the optic vesicle and the midline region; we specifically follow those originating from the midline region. These cells exit the midline, initially exhibiting a bipolar morphology, however, they lose their bipolar morphology and exhibit a variety of morphologies within the optic stalk, none of which are observed in the wild type optic stalk. Maximum intensity projection of donor cells (magenta, fluorescent dextran lineage tracer) superimposed on average projection of host membrane label (green, Tg(bactin2:EGFPCAAX)). Time interval between stacks, 9.22 minutes. Dorsal view.