Movie 4

Segmentation of ptch2^tc294z mutant optic cup to visualize disrupted optic fissure morphology. Lateral view of 3-dimensional volume rendering of manually segmented optic cup (teal), lens (gray), and optic stalk (gold), 24 hpf. The optic fissure has not formed correctly; there are no obvious, closely apposed margins in the ventral optic cup.