Movie 1

Notch activation during the specification process into pdgfrb^high MCs. Time-lapse confocal images of GFP (green) and RFP (red) fluorescence in the ISV region of Tg(TP1:GFP);TgBAC(pdgfrb:GAL4FF);Tg(4xUAS:RFP) from 42.6-80 hpf. Dorsal to the top, ventral to the bottom, anterior to the left. Images were obtained every 12 min and the relative time is shown on the bottom (hr:min). Arrows indicate the MCs with TP1:GFP and show up when TP1:GFP starts to be detected in pdgfrb+ cells, even though GFP expression in the cell above is faint at the beginning. Note that pdgfrb+ cells started to extend processes and migrate, or proliferate after the onset of TP1:GFP expression. While, pdgfrb+ cells without TP1:GFP expression reduced their fluorescence.