Movie 1

Notch activation during the specification process into pdgfrbhigh MCs. Time-lapse confocal images of GFP (green) and RFP (red) fluorescence in the ISV region of Tg(TP1:GFP);TgBAC(pdgfrb:GAL4FF);Tg(4xUAS:RFP) from 42.6-80 hpf. Dorsal to the top, ventral to the bottom, anterior to the left. Images were obtained every 12 min and the relative time is shown on the bottom (hr:min). Arrows indicate the MCs with TP1:GFP and show up when TP1:GFP starts to be detected in pdgfrb+ cells, even though GFP expression in the cell above is faint at the beginning. Note that pdgfrb+ cells started to extend processes and migrate, or proliferate after the onset of TP1:GFP expression. While, pdgfrb+ cells without TP1:GFP expression reduced their fluorescence.

Peri-arterial specification of vascular mural cells from naïve mesenchyme requires Notch signaling

Koji Ando, Weili Wang, Di Peng, Ayano Chiba, Anne K. Lagendijk, Lindsey Barske, J. Gage Crump, Didier Y. R. Stainier, Urban Lendahl, Katarzyna Koltowska, Benjamin M. Hogan, Shigetomo Fukuhara, Naoki Mochizuki, and Christer Betsholtz

Development 2019. 146:None-None; doi: 10.1242/dev.165589