Movie 1

Taz is enriched in the MC precursor before β-catenin apical enrichment and morphological changes of the cell (A-B) Animated confocal stacks of wwtr1^+/+ whole-mount follicles at the stage II (A, also shown in Fig. 5L-O) and early stage III (B, also shown in Fig. 5P-S) of oogenesis stained with Taz (magenta) and β-catenin (green) antibodies, and DAPI (blue). When Taz starts to be enriched (A, upper panel), the MC precursor is indistinguishable from neighbouring FCs in terms of shape, nuclear morphology and β-catenin localization. Once Taz levels increase in the MC precursor (B, lower panel), β-catenin is enriched at the contact point between the microvilli and the MC precursor (solid white arrow, in B) with still no obvious changes in the cell shape and nuclear morphology. The microvilli appear as densely-organized, β-catenin-positive spots in A and B. Scale Bar: 10 μm.