To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 1 Confocal stack of overexpressed Hh secreted to the cell surface of transfected S2 cells. Cellular staining and imaging are described in Supplement Figure 3. Primary α-Hh antibodies (1:250) and Cy5-labelled secondary antibodies (1:600) were used. Disrupting Hedgehog Cardin–Weintraub sequence and positioning changes cellular differentiation and compartmentalization in vivo Philipp Kastl, Dominique Manikowski, Georg Steffes, Sabine Schürmann, Shyam Bandari, Christian Klämbt, and Kay Grobe Development 2018. 145:None-None; doi: 10.1242/dev.167221 Download Original Video