Movie 1

Confocal stack of overexpressed Hh secreted to the cell surface of transfected S2 cells. Cellular staining and imaging are described in Supplement Figure 3. Primary α-Hh antibodies (1:250) and Cy5-labelled secondary antibodies (1:600) were used.

Disrupting Hedgehog Cardin–Weintraub sequence and positioning changes cellular differentiation and compartmentalization in vivo

Philipp Kastl, Dominique Manikowski, Georg Steffes, Sabine Schürmann, Shyam Bandari, Christian Klämbt, and Kay Grobe

Development 2018. 145:None-None; doi: 10.1242/dev.167221