Movie 3

Injection of plekhg5 SB-MO into the marginal zone of all 4 blastomeres at the 4-cell stages results in minimal blastopore lip formation during gastrulation. The embryos nonetheless accomplish blastopore closure when control siblings reach the neurula stages. Convergent extension tissue movements seem to drive the blastopore closure in the morphant embryos. Top two embryos are the controls and the bottom two embryos are the morphants.

The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation

Ivan K. Popov, Heather J. Ray, Paul Skoglund, Ray Keller, and Chenbei Chang

Development 2018. 145:None-None; doi: 10.1242/dev.168922