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Cranial neural tube closure is restored by combination of TPEN and zinc supplementation.
Culture of a 9 somite VenMyr mouse embryo with both 15μM TPEN and 25μM zinc allows cranial neural tube closure although neural tube closure was delayed to 14hr 48min±3hr 33min (n=6).

Zinc deficiency causes neural tube defects through attenuation of p53 ubiquitylation

Huili Li, Jing Zhang, and Lee Niswander

Development 2018. 145:None-None; doi: 10.1242/dev.169797