Movie 1

Live cell imaging of single fertilization of the central cell caused by DMP9KD sperm cells
Tiny intense signals and a larger circle signal are DMP9KD sperm nuclei and egg cell nucleus, respectively. One of the DMP9KD sperm signal moved to the central cell position, whereas another sperm signal remained at near the egg cell nucleus.

The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants

Taro Takahashi, Toshiyuki Mori, Kenji Ueda, Lixy Yamada, Shiori Nagahara, Tetsuya Higashiyama, Hitoshi Sawada, and Tomoko Igawa

Development 2018. 145:None-None; doi: 10.1242/dev.170076