Movie 2

Live cell imaging of delayed fertilization of the egg cell caused by DMP9^KD sperm cells
Tiny intense signals and a larger circle signal are DMP9^KD sperm nuclei and egg cell nucleus, respectively. The time lag of the movement between 1st and 2nd sperms was about 1.5 hours.

The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants

Taro Takahashi, Toshiyuki Mori, Kenji Ueda, Lixy Yamada, Shiori Nagahara, Tetsuya Higashiyama, Hitoshi Sawada, and Tomoko Igawa

Development 2018. 145:None-None; doi: 10.1242/dev.170076

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