Movie 2

Live cell imaging of delayed fertilization of the egg cell caused by DMP9KD sperm cells
Tiny intense signals and a larger circle signal are DMP9KD sperm nuclei and egg cell nucleus, respectively. The time lag of the movement between 1st and 2nd sperms was about 1.5 hours.

The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants

Taro Takahashi, Toshiyuki Mori, Kenji Ueda, Lixy Yamada, Shiori Nagahara, Tetsuya Higashiyama, Hitoshi Sawada, and Tomoko Igawa

Development 2018. 145:None-None; doi: 10.1242/dev.170076