Movie 10

Acute inhibition of aPKC leads to loss of any asymmetric localization of Mira when apkc^as4 mutant larval NBs cycle into colcemid induced mitotic arrest. in Example of an isolated apkc^as4 NB expressing Baz::GFP and Mira::mCherry. Left Panel shows Miranda (Grey). Right Panel shows Miranda (Cyan) and Baz (Red). NB enters mitosis in the presence of colcemid and 10μM 1NA-PP1. Z stacks collected every 2min, movie displayed at 5 frames per sec Time: hh:mm. Scale bar: 10μm.