Movie 11

Control larval NBs in primary cell culture polarize Mira normally when cycling into colcemid induced arrest in the presence of 1NA-PP1. Example of an isolated control NB expressing Baz::GFP and Mira::mCherry. Left Panel shows Miranda (Grey). Right Panel shows Miranda (Cyan) and Baz (Red). NB enters mitosis in the presence of colcemid and 10μM 1NA-PP1. Z stacks collected every 2min, movie displayed at 5 frames per sec. Time: hh:mm. Scale bar: 10μm.