Movie 12

Basal Mira localization is unaffected by 1NA-PP1 addition in colcemid arrested control larval NBs upon addition of 1NA-PP1. Example of an isolated control NB expressing Baz::GFP and Mira::mCherry. Left Panel shows Miranda (Grey). Right Panel shows Miranda (Cyan) and Baz (Red). NB arrested in mitosis with Colcemid prior to the addition of 10μM 1NA-PP1. Z stacks collected every 2min, movie displayed at 5 frames per sec. Time: hh:mm. Scale bar: 10μm.