Movie 14

The basal bias of Mira localization upon acute aPKC inhibition in colcemid arrested larval NBs in primary cell culture is Lat-A sensitive. Example of an isolated apkc^as4 NB expressing Baz::GFP and Mira::mCherry. Left Panel shows Miranda (Grey). Right Panel shows Miranda (Cyan) and Baz (Red). NB arrested in mitosis with Colcemid prior to the addition of 10μM 1NA-PP1. Latrunculin-A was added after 45minutes. Z stacks collected every 2min, movie displayed at 5 frames per sec. Time: hh:mm. Scale bar: 10μm.