Acute inhibition of aPKC leads to apical constriction of eye discs and the neuroepithelium in whole mount brains. Whole mount apkcas4 mutant brain expressing Baz::GFP and Mira::mCherry (not shown) prior and after addition of 10μM 1NA-PP1. Z stacks collected every 3min, movie displayed at 7 frames per second. 0′ marks the addition of 10μM 1NA-PP1. Time hh:mm.