Embryonic apkcas4 mutant NB polarizes Baz and Mira normally before addition of 1NA-PP1 and polarizes Baz but not Mira after 1NA-PP1 addition. Example of a aPKCas4 embryonic NB expressing Baz::GFP (green) and Mira::mCherry (magenta). Z stack collected every 2min30sec, movie displayed at 3 frames per sec. Time: mm:ss. 00:00 marks the addition of 20 μM 1NA-PP1. Scale bar: 5 μm.