Time-lapse ex vivo of a wing imaginal disc after irradiation. Nuclei marked in green indicate DBS-S activation, LifeAct-Ruby shows actin in red. The time-lapse is showing an apical focal plane of the wing disc. Notice the progressive accumulation of GFP-positive nuclei in apical focal planes. The recording started 90 minutes after irradiation and it lasted for 40 minutes. Images were acquired within a 2 minutes interval.