Movie 6

Tracking of apoptotic larval epithelial cells (LECs) in the fly abdomen cells during pupal stages with DBS-S. In non-dying cells the GFP signal from DBS-S is retained at the cellular membranes (arrowhead point ER-Glogi structures), remaining the nuclei unlabelled. Arrow highlights a dying LEC that progressively accumulates the GFP signal into the nucleus. Strong nuclear GFP signal is only distinguishable 20 minutes after movie starts, when presumably high levels of caspase activation are achieved. Nuclear accumulation of GFP is correlated with the concomitant depletion of the signal from the cytoplasm. At the end of the movie, the nucleus of the apoptotic LEC is fragmented. A region of a hemi-segment of segment A2 is shown. The time-length of the movie was 2.6 hours and frames were acquired every 150 seconds. Anterior is to the left. Dorsal is up.