Movie 7

Tracking of apoptotic histoblasts in the fly abdomen during pupal stages with DBS-S. In non-dying histoblasts the GFP signal from DBS-S is retained at the cellular membranes and the nuclei remain unlabelled. Arrows highlight dying histoblasts that progressively accumulates the GFP signal into the nucleus. Eventually the nucleus of the apoptotic cells is fragmented and the dying/dead cell delaminates. A region of a hemi-segment of segment A2 is shown. The time-length of the movie was 52.5 minutes and frames were acquired every 150 seconds. Anterior is to the left. Dorsal is up.