Reduction of Arp2 or Wave function suppresses de novo branch formation. (Related to Fig. 2, to Fig. 3 and to Fig. S2)
Time-lapse imaging of class IV ddaC neurons of 2nd instar larvae expressing control, Arp2 or wave RNAi constructs and UASmCD8GFP to highlight dendrite morphology. Images were obtained every 5 minutes over a 30 minutes recording time. Arrows point to representative events of de novo terminal branch formation. De novo branch formation events are clearly reduced upon Arp2 or wave RNAi. In addition the remaining branches are less dynamic. Scale bar = 50 μm.
Tomke Stürner, Anastasia Tatarnikova, Jan Mueller, Barbara Schaffran, Hermann Cuntz, Yun Zhang, Maria Nemethova, Sven Bogdan, Vic Small, and Gaia Tavosanis
Development
2019. 146:None-None; doi: 10.1242/dev.171397