Movie 4

Local activation of PA-Rac1CA promotes branchlet formation. (Related to Fig. 3)
Time-lapse recording of a ROI of a ddaC class IV neuron of a third instar larva expressing the photoactivatable constitutive active Rac1 construct PA-Rac1CA [genotype ppk-Gal4/UASmCherry PA Rac1]. Localized photoactivation within the shown region of interest was obtained with a 5 mW 458 nm laser. UAS-mCherry was imaged every minute over a 40 minutes period. After photoactivation single branchlets and groups of branchlets abundantly emerged from the existing dendrite branches (arrows). Scale bar = 10 μm.