Movie 5

Local activation of PA-Rac1DN does not promote branchlet formation. (Related to Fig. 3)
Time-lapse recording of a ROI of a ddaC class IV neuron of a third instar larva expressing the photoactivatable dominant-negative Rac1 construct PA-Rac1DN [genotype ppk-Gal4/ UASmCherry PA Rac1DN]. Localized photoactivation within the region of interest was obtained with a 5 mW 458 nm laser. UAS-mCherry was imaged every minute over a 40 minutes period. Minimal formation of filopodia was observed after photoactivation. Scale bar = 10 μm.