Movie 6

wave RNAi suppresses filopodium formation after local activation of PA-Rac1CA. (Related to Fig. 3)
Time-lapse recording of a ROI of a ddaC class IV neuron of a third instar larva expressing the photoactivatable constitutive active Rac1 construct PA-Rac1CA in the background of cell-autonomous wave RNAi [genotype wave RNAi / UAS-mCherry PA Rac1CA; ppk-Gal4]. Localized photoactivation within the region of interest was obtained with a 5 mW 458 nm laser. UAS-mCherry was imaged every minute over a 40 minutes period. The abundant formation of filopodia by PA Rac1 CA is largely suppressed upon wave RNAi. Scale bar = 10 μm.