Movie 5

MMP migration is impaired in embryos with different combinations of six1/six4 mutations. Time-lapse images of muscle growth and migration in (A) a wild-type embryo, (B) a Δsix1b;4b homozygote, (C) a Δsix1b;4b homozygote, also heterozygous for Δsix1a;4a, and (D) a Δsix1a;4a;Δsix1b;4b mutant. Images were taken every five minutes from 31 to 75 hpf. All embryos express six1b:lyn-GFP (green) transgene and the slow muscle transgene, smyhc1:lyn-tdTomato (magenta). Each panel is representative of at least two imaged embryos. Scale bar is 100 μm and applicable to all panels.