Movie 7

The AbFM is particularly affected by cyclopamine treatment. Confocal stack through embryos carrying six1b:lyn-GFP (mint), and mylpfa:mCherry (blue) that had been injected with mRNA encoding H2B-CFP (magenta); the fin bud outlines (yellow circles) are based on H2B-CFP label. Compared to (A) ethanol-treated control, the (B) cyclopamine-treated embryo has a small fin bud, with fewer MMPs than control. The difference is particularly apparent at the distal edge of the fin bud, where a large gap is present in the cyclopamine-treated embryo. Images in the confocal stack are spaced one micron apart. Scale bar is 100 μm.