Movie 4
Immunocytochemical analysis reveals non-uniform distribution of opsins in a stage 3 organoid. 3D reconstruction of a multiphoton z stack showing the surface of a stage 3 (d241) retinal organoid, with expression of M/L OPSIN (red) and ROD OPSIN (green) relative to nuclei (blue).
Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines
Development 2019. 146:None-None; doi: 10.1242/dev.171686