Movie 4

Immunocytochemical analysis reveals non-uniform distribution of opsins in a stage 3 organoid. 3D reconstruction of a multiphoton z stack showing the surface of a stage 3 (d241) retinal organoid, with expression of M/L OPSIN (red) and ROD OPSIN (green) relative to nuclei (blue).

Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines

Elizabeth E. Capowski, Kayvan Samimi, Steven J. Mayerl, M. Joseph Phillips, Isabel Pinilla, Sara E. Howden, Jishnu Saha, Alex D. Jansen, Kimberly L. Edwards, Lindsey D. Jager, Katherine Barlow, Rasa Valiauga, Zachary Erlichman, Anna Hagstrom, Divya Sinha, Valentin M. Sluch, Xitiz Chamling, Donald J. Zack, Melissa C. Skala, and David M. Gamm

Development 2019. 146:None-None; doi: 10.1242/dev.171686