Movie 5

Immunocytochemical analysis highlighting elongated photoreceptor outer segments in an advanced stage 3 organoid. 3D reconstruction of a multiphoton z stack showing the surface of an older stage 3 (d345) retinal organoid, with expression of M/L OPSIN (red) and ROD OPSIN (green) relative to nuclei (blue).

Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines

Elizabeth E. Capowski, Kayvan Samimi, Steven J. Mayerl, M. Joseph Phillips, Isabel Pinilla, Sara E. Howden, Jishnu Saha, Alex D. Jansen, Kimberly L. Edwards, Lindsey D. Jager, Katherine Barlow, Rasa Valiauga, Zachary Erlichman, Anna Hagstrom, Divya Sinha, Valentin M. Sluch, Xitiz Chamling, Donald J. Zack, Melissa C. Skala, and David M. Gamm

Development 2019. 146:None-None; doi: 10.1242/dev.171686