Movie 7

Stage 3 organoids contain inner and outer nuclear layers with appropriate cell types. 3D reconstruction of a confocal z stack of a thick section from a stage 3 retinal organoid showing expression of cone ARR3 (red), ROD OPSIN (yellow) and VSX2 (bipolar cells; green) relative to nuclei (blue). Note the presence of pedicles associated with the ARR3+ cone axons vs. spherules associated with the ROD OPSIN+ rod axons present within the ONL of this organoid.