Movie 3
Related to Figure 4a. Peripodial cells remain cohesive despite deterioration of the matrix layer.
Time-lapse confocal images of the peripodial epithelium of a leg disc (dorsal view) expressing GFP-tagged collagen (vkg-GFP) and GFP-tagged E-cadherin (E-Cad-KI[GFP]).Top: matrix plane; middle: cell-cell junction plane; bottom: merged images (matrix plane: cyan, cell-cell junction plane: magenta). Image stacks were acquired every 3.75 min.
Physical and functional cell-matrix uncoupling in a developing tissue under tension
Development 2019. 146:None-None; doi: 10.1242/dev.172577