Related to Figure 5a. Uncoupling of the ECM from the cell monolayer during peripodial epithelium opening.
Time-lapse confocal images (z-projections) of a leg disc expressing fluorescent collagen (vkg-GFP, left) and myosin (sqh-TagRFPt[9B], center) during phases I-III. Right: merged images (cyan: collagen, magenta: myosin). Image stacks were acquired every 7.5 min.