Time-lapse imaging of Dictyostelium aggregation showing the behaviour of cells with spontaneous DNA damage. High Rad51-Neon expressing cells can be tracked from the onset of differentiation, until late aggregation and slug departure. The high Rad51 cells appear to remain at the edge of cell aggregates. All cells express the red nuclear marker (H2Bv3-mCherry). Time after starvation onset is shown.