To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 2 Cytoplasmic nucleation of MVIs. Maximum projection of ERM-1::mNG expressing worms silenced for V0/vha-1 during 96h. Arrowheads and arrows indicate irregular MVIs/vacuoles and cytoplasmic nucleating MVIs, respectively. A V0-ATPase-dependent apical trafficking pathway maintains the polarity of the intestinal absorptive membrane Aurélien Bidaud-Meynard, Ophélie Nicolle, Markus Heck, Yann Le Cunff, and Grégoire Michaux Development 2019. 146:None-None; doi: 10.1242/dev.174508 Download Original Video