Movie 16

Time-lapse confocal microscopy of GFP-NMY-2, mCherry-PAR-2 at the equatorial plane in air-1 (RNAi) in one-cell stage C. elegans embryos. Time-lapse movies were acquired using FV3000 Confocal system, 60X 1.4 NA objective, with high-sensitivity cooled GaAsP detection unit (Olympus Corporation, Japan) at 1 frame every 10 seconds with the z-step size of 0.5 μm covering a thickness of 3-3.5 μm. Maximum z-projection is shown, played at 20 frames/sec. Note the very minute clearing of GFP NMY-2 puncta from the anterior and posterior cortex in air-1 (RNAi) embryo, at the time of polarity establishment, in contrast to control embryo (Movie 15).