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Movie 17

Time-lapse confocal microscopy of GFP-NMY-2, mCherry-PAR-2 at the cortical plane in control one-cell stage C. elegans embryos. Time-lapse movies were acquired using FV3000 Confocal system, 60X 1.4 NA objective, with high-sensitivity cooled GaAsP detection unit (Olympus Corporation, Japan) at 1 frame every 10 seconds, played at 20 frames/sec. Compare with Movie 9 (control). Note the appearance of PAR-2 polarity axis is coincident with NMY-2 exclusion from the posterior cell cortex at the time of polarity establishment.