Movie 19
Time-lapse confocal microscopy of GFP-PGL-1 in control one-cell stage of C. elegans embryos. Time-lapse movies were acquired using FV3000 Confocal system, 60X 1.4 NA objective, with high-sensitivity cooled GaAsP detection unit (Olympus Corporation, Japan) at 1 frame every 20 seconds, with 1 μm step size covering a z-thickness of 7 μm. Maximum z-projection is shown, played at 20 frames/sec.
Centrosome Aurora A regulates RhoGEF ECT-2 localisation and ensures a single PAR-2 polarity axis in C. elegans embryos
Development 2019. 146:None-None; doi: 10.1242/dev.174565