Movie 27

Time-lapse confocal microscopy of GFP-TAC-1, mCherry-PAR-2 in posteriorly-fertilized [p.f] control embryo. Time-lapse movies were acquired using FV3000 Confocal system, 60X 1.4 NA objective, with high-sensitivity cooled GaAsP detection unit (Olympus Corporation, Japan) at 1 frame every 15-30 s, with 1 μm step size covering a z-thickness of 7-11 μm. Maximum intensity projection is shown, played at 20 frames/sec. Note the positive correlation between the position of the centrosome and the site of establishment of mCherry-PAR-2 polarity axis in control embryo, undergoing p.f.