Movie 44

Time-lapse confocal microscopy of GFP-ECT-2, mCherry-PAR-2 in zyg-9 (RNAi) in one-cell stage C. elegans embryos. Time-lapse movies were acquired using FV3000 Confocal system, 60X 1.4 NA objective, with high-sensitivity cooled GaAsP detection unit (Olympus Corporation, Japan) at 1 frame every 10 seconds, played at 20 frames/sec. Note the exclusion of ECT-2 and the appearance of a single PAR-2 polarity axis at the posterior cortex in this embryo, as in control embryo (Movie 37).