Movie 1

In vivo time-lapse imaging shows clearance of PI⁺ cells and appearance of pyknotic nuclei after brain injury. The movie was recorded in an H2A:GFP animal in the presence of PI from 120 to 230 min after injury. PI⁺ cells (magenta arrows) are removed, while pyknotic nuclei (white arrows) progressively appear within this time window. Scale bar, 15 μm.