Movie 1

Tracking of dorsal pericardial cells. 3D reconstruction of an in vivo time-lapse imaging of epi:GFP; myl7:mRFP 52 hpf embryo during 18 h. Shown is a ventral view. GFP labels dorsal pericardium (DP) and proepicardium (PE) (green) and mCherry labels the myocardium of the heart tube in red. From frame 6 onwards, the DP was isolated and shown in a top 2D view. Blue dots indicate tracked DP cells, overall tracks are shown in frame 62 and 92; in frame 68, white arrows indicate the direction of the movement of tracked cells. Scale bar: 50 μm. Shown is a representative movie from ≥3 biological and ≥ 2 technical replicates.

Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells

Laura Andrés-Delgado, Alexander Ernst, María Galardi-Castilla, David Bazaga, Marina Peralta, Juliane Münch, Juan M. González-Rosa, Inês Marques, Federico Tessadori, José Luis de la Pompa, Julien Vermot, and Nadia Mercader

Development 2019. 146:None-None; doi: 10.1242/dev.174961