Movie 13

2,3-Butanedione monoxime (BDM) inhibits F-actin accumulation at the dorsal pericardial midline. 3D reconstruction of in vivo time-lapse imaging of an epi:GFP;β-actin:LifeAct-RFP embryo starting at 52 hpf. The embryo was treated with 10 mM butanedione monoxime during acquisition. GFP-labeled dorsal pericardium (DP) and proepicardial (PE) cells are shown in green. LifeAct-RFP marking F-actin is shown in red. The ventral pericardium was digitally clipped out to allow a better view on the DP. On the left merged channels are shown. Scale bar: 50 μm. Shown is a representative movie from ≥ 3 biological and ≥ 2 technical replicates.

Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells

Laura Andrés-Delgado, Alexander Ernst, María Galardi-Castilla, David Bazaga, Marina Peralta, Juliane Münch, Juan M. González-Rosa, Inês Marques, Federico Tessadori, José Luis de la Pompa, Julien Vermot, and Nadia Mercader

Development 2019. 146:None-None; doi: 10.1242/dev.174961